A vitamin C test using High-Performance Liquid Chromatography (HPLC) analyzes vitamin C (ascorbic acid) levels in a serum or heparinized plasma sample to assess nutritional status or identify deficiency. The process involves sample preparation to remove interfering substances, followed by separation of vitamin C on a reverse-phase column and detection by UV or electrochemical detectors. Results help in managing conditions like scurvy, assessing immune health, and guiding dietary recommendations.
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